Identification of cell type-specific gene expression profiles

Triple immunolocalization
Triple immunolocalization PIN1, PIN2, PIN4, Arabidopsis thaliana, F. Ditengou and K. Palme, Freiburg

Cell fate regulation in the embryo gives rise to cells with different identities. These individual cell types can be distinguished on the basis of their expression of specific groups of genes (transcriptomes).

In the project, such cell-specific transcriptomes, are identified by Fluorescence Activated Cell Sorting (FACS) of plant lines expressing fluorescent proteins in one or another cell type, followed by microarray hybridization to determine which genes are expressed in early embryogenesis.

Transcription factors controlling cell type-specific expression are identified by yeast one-hybrid screening of promoter regions from cell-type specific genes. In many instances, newly identified regulators and their targets are investigated using High-Throughput mRNA In-Situ Hybridization (HT-ISH), and their functions are addressed using reverse genetic methods.

Trainee’s Contribution

  • PhD-student hosted in Gerd Jürgens’ lab at the University of Tübingen/Germany.
  • PhD-student hosted in Thomas Laux’ lab at the Albert-Ludwigs Universität Freiburg/Germany.
  • Post-doc hosted at Intavis AG, Cologne/Germany.

Involvement

Martin Hobe
Gerd Jürgens
Thomas Laux
Ben Scheres