Dissection of transcriptional regulation of cell type specific gene expression

Apical cells
Apical cells of the 16 cell embryo enter the stem cell niche fate by expression the homeobox gene WUSCHEL, Tucker et al. Development 135, 2839-2843, Fig. 4A.

The network has preliminarily identified key regulators that act as crucial determinants for different cell fates, which arise after division of the plant zygote. The cell type specific gene expression of these key regulators reveals the dynamics of cell fate regulatory components in early embryogenesis. Additionally the identification of their upstream regulators show which factors set up the initial partitioning of gene expression domains that starts off embryogenesis.

SPIM microscopy is used to visualize cell fate regulators in live embryos. For functional studies of transcription factors the promoters of specific cell fate regulator genes are first dissected and the cis-elements important in early embryogenesis are identified. Next the transcription factors binding to cis-elements are identified and characterized.

It is anticipated that these studies will divulge not only new factors but also cross-regulatory events between the key regulatory genes preliminarily identified for this project.

Trainee’s Contribution

  • PhD-student hosted in Thomas Laux’ lab at the Albert-Ludwigs Universität Freiburg/Germany.
  • PhD-student hosted in Ben Scheres’ lab at the Universiteit Utrecht/The Netherlands.
  • Post-doc hosted in Thomas Laux’ lab at the Albert-Ludwigs Universität Freiburg/Germany.

Involvement
Thomas Laux
Ben Scheres